Characterization of sulfhydryl oxidase from Aspergillus tubingensis
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of sulfhydryl oxidase from bovine milk.
A method is described for purification of sulfhydryl oxidase from bovine milk which consistently yields preparations with greater than 3000-fold purification over skim milk. A concentration-dependent association-dissociation of the enzyme was adapted to the development of an isolation procedure. Purified preparations exhibited two zones, both of which displayed activity, upon polyacrylamide dis...
متن کاملInfectious keratitis caused by Aspergillus tubingensis.
PURPOSE To report 2 cases of keratomycosis caused by Aspergillus tubingensis. METHODS The therapeutic courses were recorded for 2 male patients, 52 and 78 years old, with fungal keratitis caused by black Aspergillus strains. Morphological examination of the isolates was carried out on malt extract agar plates. A segment of the beta-tubulin gene was used for molecular identification. Antifunga...
متن کاملRaw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
BACKGROUND Starch is one of the most abundant organic polysaccharides available for the production of bio-ethanol as an alternative transport fuel. Cost-effective utilisation of starch requires consolidated bioprocessing (CBP) where a single microorganism can produce the enzymes required for hydrolysis of starch, and also convert the glucose monomers to ethanol. RESULTS The Aspergillus tubing...
متن کاملInfluences of sulfhydryl oxidase isolated from Aspergillus niger on physicochemical properties of starch and rheological properties of wheat dough
The sulfhydryl oxidase (SHOxase; EC. 1.8.3.2) which catalyzes the oxidation of sulfhydryl groups to disulfides was purified from Aspergillus niger using a combination of chromatographies. The influence of SHOxase on starch properties was tested by rheometer, farinographand scanning electron microscopy (SEM). Moreover, the gelatinization and retrogradation were studied by differential scanning c...
متن کاملAspergillus tubingensis and Aspergillus niger as the dominant black Aspergillus, use of simple PCR-RFLP for preliminary differentiation.
This work aimed to identify the species distribution of common clinical and environmental isolates of black Aspergilli based on simple restriction fragment length polymorphism (RFLP) analysis of the β-tubulin gene. A total of 149 clinical and environmental strains of black Aspergilli were collected and subjected to preliminary morphological examination. Total genomic DNAs were extracted, and PC...
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ژورنال
عنوان ژورنال: BMC Biochemistry
سال: 2017
ISSN: 1471-2091
DOI: 10.1186/s12858-017-0090-4